EquationsandNomogramfor the Relationshipof HumanBloodP50to 2,3-Diphosphoglycerate, C02, and H
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چکیده
We describe a new method for tonometry of small amounts of blood (up to 0.25 mL) at known Po2 Pco2 and temperature, in small, reusable, closed Pyrex flasks. Equilibrated blood is analyzed for oxygen saturation, pH, and organic phosphate concentration with standard techniques, and its (the Po2at which hemoglobin is half-saturated with oxygen) is determined with full control of all the variables known to affect it. The SD in the measurement of P50 is 0.044 kPa (0.33 mmHg). We made 63 determinations of P50 on normal human blood under different conditions of pH and Pco2 and with different concentrations of 2,3diphosphoglycerate and AlP. Empirical equations and a nomogram were derived, which allow the calculation of P50 from known values of Pco2 pH, and [2,3-DPG]/[Hb4] molar ratio with a SD of 97 and 114 Pa (0.73 and 0.86 mmHg), respectively.
منابع مشابه
The interaction of 2,3-diphosphoglycerate with human hemoglobin. Effects on the alkaline and acid Bohr effect.
A model is presented which is able to describe satisfactorily the increase of the alkaline and acid Bohr effect due to the binding by human hemoglobin of 2,3-diphosphoglycerate. From an analysis of this extra Bohr effect it could be concluded that the histidines H21(143)/3 are very probably responsible for the increase in alkaline Bohr effect. The second ionization of the phosphate groups of 2,...
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